- Mass spectrometry (MS) is an analytical technique for the determination of the elemental composition of a sample or molecule. It is also used for elucidating the chemical structures of molecules, such as peptides and other chemical compounds. The MS principle consists of ionizing chemical compounds to generate charged molecules or molecule fragments and measurement of [...]
- In 1886, Eugen Goldstein observed rays in gas discharges under low pressure that traveled away from the anode and through channels in a perforated cathode, opposite to the direction of negatively charged cathode rays (which travel from cathode to anode). Goldstein called these positively charged anode rays “Kanalstrahlen”; the standard translation of this term into [...]
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- Mass Spectrometry Methods
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Plasma Source Mass Spectrometry: The New Millennium (Special Publications, Number 267)
Plasma Source Mass Spectrometry: The New Millennium (Special Publications, Number 267)
Covers a diverse range of topics including the fate of the sample as it passes through the sample introduction system, chemical resolution using reaction and collision cells, and the results of analyses ranging from natural waters and archaeological isotope ratios to organometallic speciation in biological materials.
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Improved analysis of membrane protein by PVDF-aided, matrix-assisted laser desorption/ionization mass spectrometry [An article from: Analytica Chimica Acta]
Improved analysis of membrane protein by PVDF-aided, matrix-assisted laser desorption/ionization mass spectrometry [An article from: Analytica Chimica Acta]
This digital document is a journal article from Analytica Chimica Acta, published by Elsevier in 2006. The article is delivered in HTML format and is available in your Amazon.com Media Library immediately after purchase. You can view it with any web browser.
Description:
Characterization of membrane proteins remains an analytical challenge because of difficulties associated with tedious isolation and purification. This study presents the utility of the polyvinylidene difluoride (PVDF) membrane for direct sub-proteome profiling and membrane protein characterization by matrix-assisted laser desorption/ionization mass spectrometry (MALDI-MS). The hydrophobic adsorption of protein, particularly membrane proteins, on the PVDF surface enables efficient on-PVDF washing to remove high concentrations of detergents and salts, such as up to 5% sodium dodecyl sulfate (SDS). The enhanced spectrum quality for MALDI detection is particularly notable for high molecular weight proteins. By using on-PVDF washing prior to MALDI detection, we obtained protein profiles of the detergent-containing and detergent-insoluble membrane fractions from Methylococcus capsulatus (Bath). Similar improvements of signal-to-noise ratios were shown on the MALDI spectra for proteins electroblotted from SDS-polyacrylamide gel electrophoresis (SDS-PAGE) onto the PVDF membrane. We have applied this strategy to obtain intact molecular weights of the particulate methane monooxygenase (pMMO) composed of three intrinsic membrane-bound proteins, PmoA, PmoB, and PmoC. Together with peptide sequencing by tandem mass spectrometry, post-translational modifications including N-terminal acetylation of PmoA and PmoC and alternative C-terminal truncation of PmoB were identified. The above results show that PVDF-aided MALDI-MS can be an effective approach for profiling and characterization of membrane proteins.
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Mass Spectrometry of Biological Materials, Second Edition
Mass Spectrometry of Biological Materials, Second Edition
This timely Second Edition of an established resource covers the huge expansion of information that has taken place since the publication of the first edition;providing up-to-the-minute discussions on the application of mass spectrometry to the biological sciences. Shows how and why experiments are performed and furnishes details to facilitate the duplication of results! Completely rewritten and enlarged by internationally recognized scientists from industry and academia, Mass Spectrometry of Biological Materials, Second Edition reviews the ionization methods and instrumentation used in the analysis of biological materials introduces new instrumental technology that can solve fundamental problems in biology demonstrates techniques for isolating and identifying target molecules in complex mixtures that are often present in only trace amounts describes methods for characterizing proteins at very low levels of sample material analyzes noncovalent interactions and the use of hydrogen/deuterium exchange for tertiary structure determination explains the characterization of oligonucleotides and DNA supplies in-depth coverage of screening protein databases using data generated by mass spectrometry and more! Containing over 800 bibliographic citations and illustrations, Mass Spectrometry of Biological Materials, Second Edition is a useful guide for mass spectrometrists; analytical, medicinal, and protein chemists and biochemists; biotechnologists; enzymologists; molecular biologists; agricultural scientists; and graduate-level and continuing-education students in these disciplines.
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Mass Spectrometry News
Show has largest travelling exhibit of mummies
LOS ANGELES, Calif. – A 10-month-old baby who lived in Peru 6,420 years ago and a 17th-century nobleman; a South American woman with a tattoo on each breast and one on her face, a woman who had tuberculosis, a child who had a heart condition and a youngster with [...]
LA show has largest traveling exhibit of mummies
A 10-month-old baby who lived in Peru 6,420 years ago and a 17th-century nobleman; a South American woman with a tattoo on each breast and one on her face, a woman who had tuberculosis, a child who had a heart condition and a youngster with a facial tumor.
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Revealed: Art’s forgeries, fakes and mistakes
LONDON (Reuters) – A phony Botticelli, a faux Rembrandt, a Holbein hoax alongside other forgeries, fakes and mistakes come under the microscope in the latest exhibition at London’s National Gallery.
Read more on Reuters
Mass Spectrometry Videos
RapidFire technology uses innovative microfluidics for an SPE-based solution, enabling detection of native compounds. RapidFire MS is 10 to 100 times faster than conventional MS screening methods. Requiring no labels or surrogates, RapidFire enables the screening of intractable or challenging targets which are not amenable to fluorescence or luminescence.
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Bruker GC/MS systems are the established leader for a wide range of chemical analysis applications for a diversity of samples and analytes. These GC/MS solutions are designed to deliver unmatched sensitivity, flexibility, and maximum utility all at a premium value to handle even the most challenging applications including: • Food and Product Safety Assessment • [...]
Protein modification tutorial for mMass software. mMass presents an open source cross-platform environment for precise analysis of mass spectrometric data. Available for free at www.mmass.org
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