Thursday, September 09, 2010

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Archive for July, 2010

Mass spectrometry proteomic

Mass spectrometry
Video Rating: 4 / 5

Aldehyde and ketone problems (2)

Organic chemistry: Aldehyde/ketone nomenclature problems. Spectroscopy problems. Hydration (nucleophilic addition of water to aldehydes and ketones to form geminal diols). Reactivity of aldehydes and ketones. Effects of acid or base on reactivity. Mass spectrometry of aldehydes and ketones; McLafferty rearrangement. Nucleophilic addition of thiols to aldehydes and ketones to form thioacetals; desulfurization of thioacetals with Raney nickel. This is a recording of a tutoring session, posted with the students’ permission. These videos are offered on a “pay-what-you-like” basis. You can pay for the use of the videos at my website: www.freelance-teacher.com For a printable document containing the handout discussed in this video series, go to my website. For a list of all the available video series, arranged in suggested viewing order, go to my website. For a playlist containing all the videos in this series, click here: www.youtube.com (1) Aldehyde/ketone nomenclature problems (2) Continued. Spectroscopy problems (3) Contined. Hydration (nucleophilic addition of water to aldehydes and ketones to form geminal diols) (4) Continued. Reactivity of aldehydes and ketones (5) Continued. A hydration problem (6) Continued. Effects of acid or base on reactivity (7) Continued (8) Continued. Mass spectrometry of aldehydes and ketones; McLafferty rearrangement (9) Continued (10) Continued (11) Nucleophilic addition of thiols to aldehydes and ketones to form thioacetals; desulfurization
Video Rating: 4 / 5

Mass Spectrometry and Genomic Analysis

Mass Spectrometry and Genomic Analysis

The human genome project is set to revolutionise health care and medicine in the near future. The genetic make up of each individual gives clues as to the genetic factors that predispose one to a particular genetic disease. Single Nucleotide Polymorphisms (SNPs), single base changes in the nucleotide DNA sequence of individuals, are thought to be the main cause of genetic variation. By comparing patterns of SNP allele frequencies between disease affected and control populations, disease associated SNPs can be identified and potential disease gene(s) located. These types of study necessitate genotyping of thousands of SNPs which requires the use of powerful, high throughput, systems of analysis. Mass spectrometry is fast becoming the preferred technology for this type of high throughput analysis. This book contains a collection of descriptions of some of the most outstanding advances in the field of mass spectrometry from which, I hope, the reader will be able to learn both the principles and the most up to date methods for its use in genomic analysis. It covers the general principles of the technologies and more in depth detailed descriptions for more academic reading and information. Audience: Whether you are a student, a post-doctoral researcher or experienced MS user, this book will be a stimulating addition to the analytical arena of mass spectrometry and genomic analysis.

List Price: $ 139.00

Price: $ 115.39

Selected Reaction Monitoring (SRM) – outside view

Mass spectrometry animation of SRM : – ions of 3 different masses (ie colors) start in the first quadrupole ; – one ion of a selected mass (ie blue color) is filtered in Q1 ; – then fragmented in q2 into 3 different fragments (with different masses, ie shapes) ; – one of these fragments is filtered in Q3. – this selected fragment hits the detector after Q3 and produces a signal (ie flash). Please check www.cruxgraphix.ch for details.

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