Gas Chromatography GC
Gas Chromatography GC
Last Updated on Saturday, 26 June 2010 01:08 Written by Administrator Saturday, 26 June 2010 01:08
An education video from the Royal Society of Chemistry on gas chromatography using a flame ionisation detector (FID) with a brief mention of gas chromatography mass spectrometry (GCMS). From the “Modern Instrumental Techniques for schools and colleges” DVD. For more information on the Chemistry for our Future programme please visit www.rsc.org (C) Royal Society of Chemistry
Video Rating: 4 / 5
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Tags: CHROMATOGRAPHY
Thank you
this is very useful video.thanks for nice explaining
Thank you so much …… I’ll be giving a lecture on Gas chromatography in 12 hrs … I’ll be showing this video via internet thanks a million RSC !!!!
Hi,
yes in GC the separation of analyte depends on its volatility but we should keep in mind that more polar the compound less volatile it would be,second thing the polarity of column in GC is also very important bcz inside the column the analyte molecules do not travle in a very straight line they move randomly and when they come in contact with the walls of coulmn they stay there for a while depending upon the polarity of analyte and column.
thanks alot for posting this video…i hope to get many informative videos in the future
thanks
since GC heats up the anlyte or specimen before the measurement or identification thus most organic substances like protein could not be evaluated or tested accurately or am I wrong please advice
Well polarity indeed plays an important role as it defines the extent to which a given sample molecule interacts with the given GC column. There are specific columns depending on the type of sample molecules you need to analyse. I hope this helps
This video is excellent !
polarity has more to do with hplc, gc is the simple version og heating ur small liquid sample and breaking down the constituents of ur mixture for u to idenify through retention times and area peaks
polarity has more to do with liquid chromatography compared to gc, you familiar with the saying ‘like dissolves like’ and so on, gc just heats ur liquid sample, and breaks it down for a chemist to identify constituents of a mixture through their retention times and areas.
To Traxass000
factors which will affect the retention time of the compounds are;
volatility
polarity
column temperature
column packing polarity
flow rate of the carrier gas
length of the column
Volatility will generally be the main factor influence the separation. Polarity is only important if the mixture of compounds has compounds with widely different polarities.
Good luck on your project
There are about 6 factors which will influence the retention time of the compounds in GC;
Volatility of the compound
Polarity
Column temperature
Column packing polarity
flow rate of the carrier gas
and length of the column
Volatility will have the greatest influence on the separation. Polarity is only important if you are separating compounds with widely different polarities.
Good luck on your project.
Exelente ! ;gracias ,desde buenos aires.
Really useful video
I’d be interested to see answer to traxass000 about precisely what is mean by volatility – does it refer to polarity?
Thanks for the video… It help me alot! =D
actually its more dependent on the volatility of the substances in your mixture. The more volatile compound in your mixture will be eluted (driven off) first followed by the less volatile, then lastly the least volatile one. Therefore you must know the boiling point of the substances in your mixture.
interesting
i got a question here. so GC has nothing to do with the polarity of the substances used in order to separate them (because my teacher keeps on saying the polarity plays an important roll, like the more polar particles remain behind while the rest goes through the tube) ? because here and on other videos i here people mention only volatility of the substances as an important factor. :S
need explanation asap help help :p:p its for a project and great vid btw 
Very good. thanks
Thanks, this was very useful!
thanks
thanks
thanks
thanks for posting this video. Very informative.