Measurement of tobramycin by reversed-phase high-performance liquid chromatography with mass spectrometry detection [An article from: Analytica Chimica Acta]
Measurement of tobramycin by reversed-phase high-performance liquid chromatography with mass spectrometry detection [An article from: Analytica Chimica Acta]
Last Updated on Thursday, 1 September 2011 12:11 Written by Administrator Thursday, 1 September 2011 12:11
Measurement of tobramycin by reversed-phase high-performance liquid chromatography with mass spectrometry detection [An article from: Analytica Chimica Acta]
This digital document is a journal article from Analytica Chimica Acta, published by Elsevier in 2006. The article is delivered in HTML format and is available in your Amazon.com Media Library immediately after purchase. You can view it with any web browser.
Description:
Analysis of tobramycin faces challenges owing to its significant basicity, hydrophilicity and lack of a UV absorbing chromophore. Chromatographic methods, coupled with derivatization to introduce chromophores for tobramycin analysis, were extensively studied. A direct reversed-phase HPLC method for tobramycin analysis has not been reported. Here, we would like to report a simple LC/MS method for quantitative analysis of tobramycin in pharmaceutical formulations. Reversed-phase HPLC analysis of tobramycin was achieved using a pH stable C18 column with basic (pH 11) aqueous mobile phase (ammonium hydroxide buffer), while direct detection was carried out employing a single quadruple mass detector in negative mode via electrospray ionization. This unique separation-detection combination provided simple and specific determination of tobramycin. This method was found to be linear at a tobramycin concentration range of 0.2-0.8mg/mL with a correlation coefficient value of 0.999. The quantitation limit and detection limit were calculated as 0.210 and 0.063@mg/mL, respectively, with 99.994% confidence. This method was successfully applied to measure tobramycin content in matrices containing tobramycin and other pharmaceutical formulation ingredients. Recoveries of 101.8, 97.8 and 106.7% were obtained for tobramycin spiked in the pharmaceutical formulation at concentrations of 1.68, 1.0 and 0.35mg/mL, respectively. The relative standard deviations for six injections of spiked samples ranged from 0.2 to 3.2%, indicating good method repeatability.
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Tags: Acta, Analytica, article, Chimica, CHROMATOGRAPHY, Detection, From, HighPerformance, LIQUID, Mass, Measurement, reversedphase, Spectrometry, tobramycin
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